She revealed fears that she may not be able to have children in one emotionally distressing scene from her no-holds-barred Netflix documentary.
And Lady Gaga gave fans another searingly honest glimpse into her private life in Five Foot Two, as she demonstrated how she copes with her chronic pain condition, fibromyalgia.
An access-all-areas scene showed the Grammy-award winner, 31, bending over as a female friend made an injection into her bottom.
The scene showed the fearless star pulling down her trousers and flashing her thong as she prepared for the injection.
Elsewhere in the documentary, she is seen topless while getting ready in her dressing room for her Super Bowl performance.
The singer laid her personal and professional life bare in the insightful flick, including in a distressing scene where a tearful Gaga voices one of her worst fears – not being able to have the family she so fiercely desires.
The Born This Way hit-maker, whose real name is Stefani Germanotta, sobbed as she said: ‘I don’t even know what child birth will be like.
‘I think I can get pregnant, I just don’t know – what are my hips going to do? I don’t f****** know.’
But the brave performer showed defiance in the face of her poor health, adding: I’m not afraid of those things.’
Another emotionally charged scene in the documentary, which was released on Friday, comes when the star talks about her various heart breaks.
Fans were shocked when the singer split from her long-term boyfriend model Taylor Kinney, a year and a half after they became engaged on Valentine’s Day in 2015.
In Five Foot Two she said: ‘I just want to go on tour and have a family. I never get it all right. My love life has imploded.’
Gaga also made the sad observation that her relationships often come to an end just as she hits a career milestone.
She said: ‘It’s a sad day when it’s like, I’m doing the Super Bowl and I’m so excited to do it, but I can’t help but realise that when I sold 10 million (records) I lost Matt (Williams). I sold 30 million, I lose Luc (Carl). I get the movie, I lose Taylor.
‘It’s like a turnover. This is the third time I’ve had my heart broken like this.’
Gaga announced the split to her fans in an Instagram post in July last year – where she described the couple as ‘taking a break’ before the went their separate ways permanently.
She said: ‘Taylor and I have always believed we are soulmates. Just like all couples we have ups and downs, and we have been taking a break.
‘We are both ambitious artists, hoping to work through long-distance and complicated schedules to continue the simple love we have always shared. Please root us on. We’re just like everybody else and we really love each other.